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| Miyama, Takako; Inokuma, Hisashi; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; 猪熊, 壽; 玄, 学南. |
| The clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 was examined in dogs in an area where B. gibsoni infection was endemic. Only 8 among 14 dogs with acute type B. gibsoni infection without a previous history of infection were positive. This high percentage of false-negative results is thought to be a weak point of ELISA as a diagnostic tool. However, 14 other anemic dogs with a confirmed history of B. gibsoni infection were all positive, thus confirming the higher sensitivity of ELISA in detecting a history of infection. |
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Palavras-chave: Babesia gibsoni; Diagnosis; ELISA. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/925 |
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| Miyama, Takako; Sakata, Yoshimi; Shimada, Yojiro; Ogino, Shoji; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; Nagasawa, Hideyuki; Inokuma, Hisashi. |
| To determine the distribution of Babesia gibsoni infection in dogs in the eastern part of Japan, an epidemiological survey of dogs suspected of having B. gibsoni infection was attempted using the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Thirty-five of 115 such dogs (30.4%) were positive by PCR and/or ELISA. The 35 positive dogs consisted of 28 Tosa dogs, 4 American Pit Bull Terriers, and 3 mongrel dogs in Aomori, Fukushima, Ibaraki, Gunma, Chiba, Tokyo, Kanagawa, and Nagano Prefectures. The positive dogs had a significantly lower rate of tick exposure and a higher rate of bites by other dogs. Twenty-two of 35 B. gibsoni-positive dogs were infected with hemoplasma, and the rate of infection was significantly higher than... |
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Palavras-chave: Babesia gibsoni; Eastern Japan; Enzyme-linked immunosorbent assay; Hemoplasma; Polymerase chain reaction. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/924 |
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